Kinetic responses of activated sludge to individual and joint nickel (Ni(II)) and cobalt (Co(II)): An isobolographic approach.

The effects of Ni(II) and Co(II) on the activated sludge growth rate have been assessed for a batch growth system, for a range of concentrations between 0 and 320 mg L(-1). The activated sludge was not acclimatized to the above metallic species, while a synthetic rich growth medium was used as substrate throughout out the experimental trials. Ni(II) and Co(II) have been found to stimulate microbial growth at concentrations approximately below 27 and 19 mg L(-1), with maximum stimulation concentrations 10 and 5 mg L(-1), respectively. The lethal concentrations (zero growth) for both species have been found to lie between 160 and 320 mg L(-1), with Co(II) identified as more potent growth inhibitor compared to Ni(II). The behaviour of activated sludge was also tested at the presence of three Ni(II) and Co(II) quotas, at various concentrations (75%Ni-25%Co (w/w), 50%Ni-50%Co (w/w) and 25%Ni-75%Co (w/w)). All the mixtures stimulated more drastically the activated sludge growth at relatively small concentrations, compared with the stimulation of equal concentrations of single species, whilst they also acted as more potent inhibitors at relatively high concentrations. Based on the isobole method, the data indicated that Ni(II) and Co(II) acted synergistically at the increasing stimulation and at the intoxication zones, whilst an antagonistic relation determined at the decreasing stimulation zone. Under the light of the present study, it is obvious that interactions (particularly synergism) between different metallic species should be taken into account in the methodologies used to establish criteria for tolerance levels in the environment.